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Background  RNA interference (RNAi) is a powerful method to inhibit gene expression in a sequence specific manner.    Results  Here, we described the development of RNAi by micro-injection of double-stranded RNA (dsRNA) in the pea aphid  Acyrthosiphon pisum . Injection of dsRNA into whole aphid body induced the silencing of two marker genes with different expression patterns: the ubiquitously expressed  Ap-crt  genes encoding a calreticulin and the gut specific  Ap-cath-L  gene encoding a cathepsin-L. Time-course analysis of the silencing showed similar temporal patterns for both genes: inhibition started at 1 day after injection, reached its maximum at 5 days and stopped at 7 days. A comparable 40% decrease of gene expression was observed for  Ap-crt  and  Ap-cath-L .    Conclusion  The pea aphid is the first Hemipteran insect for which genome sequence will be available soon. The gene knockdown technique developed in this study will be an essential post-genomic tool for further investigations in aphidology.

Gene knockdown by RNAi in the pea aphid Acyrthosiphon pisum