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Background  Carboxyl-terminal protease (CtpA) plays essential functions in posttranslational protein processing in prokaryotic and eukaryotic cells. To date, only a few bacterial  ctpA  genes have been characterized. Here we cloned and characterized a novel CtpA. The encoding gene,  ctpAp  ( ctpA  of  Paenibacillus lautus ), was derived from  P. lautus  CHN26, a Gram-positive bacterium isolated by functional screening. Recombinant protein was obtained from protein over-expression in  Escherichia coli  and the biochemical properties of the enzyme were investigated.    Results  Screening of environmental sediment samples with a skim milk-containing medium led to the isolation of a  P. lautus  CHN26 strain that exhibited a high proteolytic activity. A gene encoding a carboxyl-terminal protease ( ctpAp ) was cloned from the isolate and characterized. The deduced mature protein contains 466 aa with a calculated molecular mass of 51.94 kDa, displaying 29-38% amino acid sequence identity to characterized bacterial CtpA enzymes. CtpAp contains an unusual catalytic dyad (Ser 309 -Lys 334 ) and a PDZ substrate-binding motif, characteristic for carboxyl-terminal proteases. CtpAp was expressed as a recombinant protein and characterized. The purified enzyme showed an endopeptidase activity, which effectively cleaved α S1- and β- casein substrates at carboxyl-terminus as well as at multiple internal sites. Furthermore, CtpAp exhibited a high activity at room temperature and strong tolerance to conventional protease inhibitors, demonstrating that CtpAp is a novel endopeptidase.    Conclusions  Our work on CtpA represents the first investigation of a member of Family II CtpA enzymes. The gene was derived from a newly isolated  P. lautus  CHN26 strain exhibiting a high protease activity in the skim milk assay. We have demonstrated that CtpAp is a novel endopeptidase with distinct cleavage specificities, showing a strong potential in biotechnology and industry applications.

A novel carboxyl-terminal protease derived from Paenibacillus lautusCHN26 exhibiting high activities at multiple sites of substrates