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Background  Codon optimization and subcellular targeting were studied with the aim to increase the expression levels of the SAG1 78-322  antigen of  Toxoplasma gondii  in tobacco leaves. The expression of the tobacco-optimized and native versions of the  SAG1  gene was explored by transient expression from the  Agrobacterium tumefaciens  binary expression vector, which allows targeting the recombinant protein to the endoplasmic reticulum (ER) and the apoplast. Finally, mice were subcutaneously and orally immunized with leaf extracts-SAG1 and the strategy of prime boost with rSAG1 expressed in  Escherichia coli  was used to optimize the oral immunization with leaf extracts-SAG1.    Results  Leaves agroinfiltrated with an unmodified  SAG1  gene accumulated 5- to 10-fold more than leaves agroinfiltrated with a codon-optimized  SAG1  gene. ER localization allowed the accumulation of higher levels of native SAG1. However, no significant differences were observed between the mRNA accumulations of the different versions of SAG1. Subcutaneous immunization with leaf extracts-SAG1 (SAG1) protected mice against an oral challenge with a non-lethal cyst dose, and this effect could be associated with the secretion of significant levels of IFN-γ. The protection was increased when mice were ID boosted with rSAG1 (SAG1+boost). This group elicited a significant Th1 humoral and cellular immune response characterized by high levels of IFN-γ. In an oral immunization assay, the SAG1+boost group showed a significantly lower brain cyst burden compared to the rest of the groups.    Conclusion  Transient agroinfiltration was useful for the expression of all of the recombinant proteins tested. Our results support the usefulness of endoplasmic reticulum signal peptides in enhancing the production of recombinant proteins meant for use as vaccines. The results showed that this plant-produced protein has potential for use as vaccine and provides a potential means for protecting humans and animals against toxoplasmosis.

bmc biotechnology

Effect of codon optimization and subcellular targeting on Toxoplasma gondii antigen SAG1 expression in tobacco leaves to use in subcutaneous and oral immunization in mice