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Background  To characterize  Fusarium  isolates from recent cases of fungal keratitis in contact lens wearers, and to investigate fungal association with MoistureLoc solution.    Methods  We studied six fungal isolates from recent cases of keratitis in New York State. The isolates were characterized by nucleotide sequencing and phylogenetic analyses of multiple genes, and then typed using minisatellite and microsatellite probes. Experimental fungal biofilm formation was tested by standard methods. MoistureLoc solutions were tested in biofouling studies for their efficacy in elimination of  Fusarium  contamination.    Results   Fusarium solani  – corneal ulcers (2 isolates), lens case (1 isolate), and  F. oxysporum  – corneal ulcer (1 isolate), eye (1 isolate), were recovered from five patients. An opened bottle of MoistureLoc solution provided by a patient also yielded  F. solani . Two distinct genotypes of  F. solani  as well as of  F. oxysporum  were present in the isolated strains. Remarkably,  F. solani  strains from the lens case and lens solution in one instance were similar, based on phylogenetic analyses and molecular typing. The solution isolate of  F. solani  formed biofilm on contact lenses in control conditions, but not when co-incubated with MoistureLoc solution. Both freshly opened and 3-month old MoistureLoc solutions effectively killed  F. solani  and  F. oxysporum , when fungal contamination was simulated under recommended lens treatment regimen (4-hr). However, simulation of inappropriate use (15 – 60 min) led to the recovery of less than 1% of original inoculum of  F. solani  or  F. oxysporum .    Conclusion  Temporary survival of  F. solani  and  F. oxysporum  in MoistureLoc suggested that improper lens cleaning regimen could be a possible contributing factor in recent infections.

bmc ophthalmology

Molecular characterization, biofilm analysis and experimental biofouling study of Fusariumisolates from recent cases of fungal keratitis in New York State