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Background Suppression Subtractive Hybridization (SSH) is an established method for separating DNA molecules that distinguish two closely related DNA samples, which has become a powerful method for generating subtracted cDNA libraries. In this study, we postulated that SSH could be used to identify significant genetic differences between Mycobacterium tuberculosis causing meningitis and M. tuberculosis causing pulmonary tuberculosis.

bmc infectious diseases

Generation of differentially regulated genes in Mycobacterium tuberculosis isolates from cerebrospinal fluid and respiratory secretions using suppression subtractive hybridization