English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The complex responses of plants to DNA damage are incompletely understood and the role of members of the serpin protein family has not been investigated. Serpins are functionally diverse but structurally conserved proteins found in all three domains of life. In animals, most serpins have regulatory functions through potent, irreversible inhibition of specific serine or cysteine proteinases via a unique suicide-substrate mechanism. Plant serpins are also potent proteinase inhibitors, but their physiological roles are largely unknown.    Results  Six  Arabidopsis  genes encoding full-length serpins were differentially expressed in developing seedlings and mature tissues. Basal levels of  AtSRP2  (At2g14540) and  AtSRP3  (At1g64030) transcripts were highest in reproductive tissues.  AtSRP2  was induced 5-fold and  AtSRP3  100-fold after exposure of seedlings to low concentrations of methyl methanesulfonate (MMS), a model alkylating reagent that causes DNA damage. Homozygous T-DNA insertion mutants  atsrp2  and  atsrp3  exhibited no differential growth when mutant and wild-type plants were left untreated or exposed to γ-radiation or ultraviolet light. In contrast,  atsrp2  and  atsrp3  plants exhibited greater root length, leaf number and overall size than wild-type plants when exposed to MMS. Neither of the two serpins was required for meiosis. GFP-AtSRP2 was localized to the nucleus, whereas GFP-AtSRP3 was cytosolic, suggesting that they target different proteinases. Induction of cell cycle- and DNA damage-related genes  AtBRCA1 ,  AtBARD1 ,  AtRAD51 ,  AtCYCB1;1  and  AtCYCD1;1 , but not  AtATM , was reduced relative to wild-type in  atsrp2  and  atsrp3  mutants exposed to MMS.    Conclusion  Expression of specific serpin genes ( AtSRP2  and  AtSRP3  in  Arabidopsis ) is required for normal responses of plants following exposure to alkylating genotoxins such as MMS.

Serpin genes AtSRP2 and AtSRP3 are required for normal growth sensitivity to a DNA alkylating agent in Arabidopsis