English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   Coffea canephora , also called Robusta, belongs to the Rubiaceae, the fourth largest angiosperm family. This diploid species (2x = 2n = 22) has a fairly small genome size of ≈ 690 Mb and despite its extreme economic importance, particularly for developing countries, knowledge on the genome composition, structure and evolution remain very limited. Here, we report the 160 kb of the first  C. canephora  Bacterial Artificial Chromosome (BAC) clone ever sequenced and its fine analysis.    Results  This clone contains the  CcEIN4  gene, encoding an ethylene receptor, and twenty other predicted genes showing a high gene density of one gene per 7.8 kb. Most of them display perfect matches with  C. canephora  expressed sequence tags or show transcriptional activities through PCR amplifications on cDNA libraries. Twenty-three transposable elements, mainly Class II transposon derivatives, were identified at this locus. Most of these Class II elements are Miniature Inverted-repeat Transposable Elements (MITE) known to be closely associated with plant genes. This BAC composition gives a pattern similar to those found in gene rich regions of  Solanum lycopersicum  and  Medicago truncatula  genomes indicating that the  CcEIN4  regions may belong to a gene rich region in the  C. canephora  genome. Comparative sequence analysis indicated an extensive conservation between  C. canephora  and most of the reference dicotyledonous genomes studied in this work, such as tomato ( S. lycopersicum ), grapevine ( V. vinifera ), barrel medic  M. truncatula , black cottonwood ( Populus trichocarpa ) and  Arabidopsis thaliana . The higher degree of microcollinearity was found between  C. canephora  and  V. vinifera , which belong respectively to the Asterids and Rosids, two clades that diverged more than 114 million years ago.    Conclusion  This study provides a first glimpse of  C. canephora  genome composition and evolution. Our data revealed a remarkable conservation of the microcollinearity between  C. canephora  and  V. vinifera  and a high conservation with other distant dicotyledonous reference genomes. Altogether, these results provide valuable information to identify candidate genes in  C. canephora  genome and serve as a foundation to establish strategies for whole genome sequencing. Future large-scale sequence comparison between  C. canephora  and reference sequenced genomes will help in understanding the evolutionary history of dicotyledonous plants.

bmc plant biology

Microcollinearity in an ethylene receptor coding gene region of the Coffea canephora genome is extensively conserved with Vitis vinifera and other distant dicotyledonous sequenced genomes