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Abstract Background Previously, we reported effects of the <it>cry</it><it>b</it> mutation on circadian rhythms in <it>period</it> and <it>timeless</it> gene expression within isolated peripheral <it>Drosophila</it> tissues. We relied on <it>luciferase</it> activity driven by the respective regulatory genomic elements to provide real-time reporting of cycling gene expression. Subsequently, we developed a tool kit for the analysis of behavioral and molecular cycles. Here, we use these tools to analyze our earlier results as well as additional data obtained using the same experimental designs. Results Isolated antennal pairs, heads, bodies, wings and forelegs were evaluated under light-dark cycles. In these conditions, the <it>cry</it><it>b</it> mutation significantly decreases the number of rhythmic specimens in each case except the wing. Moreover, among those specimens with detectable rhythmicity, mutant rhythms are significantly weaker than <it>cry</it>+ controls. In addition, <it>cry</it><it>b</it> alters the phase of <it>period</it> gene expression in these tissues. Furthermore, peak phase of <it>luciferase</it>-reported <it>period</it> and <it>timeless</it> expression within <it>cry</it>+ samples is indistinguishable in some tissues, yet significantly different in others. We also analyze rhythms produced by antennal pairs in constant conditions. Conclusions These analyses further show that circadian clock mechanisms in <it>Drosophila</it> may vary in a tissue-specific manner, including how the <it>cry</it> gene regulates circadian gene expression.</p

Advanced analysis of a cryptochrome mutation's effects on the robustness and phase of molecular cycles in isolated peripheral tissues of Drosophila.