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Background  Pairing of homologous chromosomes at meiosis is an important requirement for recombination and balanced chromosome segregation among the products of meiotic division. Recombination is initiated by double strand breaks (DSBs) made by Spo11 followed by interaction of DSB sites with a homologous chromosome. This interaction requires the strand exchange proteins Rad51 and Dmc1 that bind to single stranded regions created by resection of ends at the site of DSBs and promote interactions with uncut DNA on the homologous partner. Recombination is also considered to be dependent on factors that stabilize interactions between homologous chromosomes. In budding yeast Hop2 and Mnd1 act as a complex to promote homologous pairing and recombination in conjunction with Rad51 and Dmc1.    Results  We have analyzed the function of the  Arabidopsis  orthologue of the budding yeast  MND1  gene (At MND1 ). Loss of At MND1  did not affect normal vegetative development but caused fragmentation and missegregation of chromosomes in male and female meiosis, formation of inviable gametes, and sterility. Analysis of the  Atmnd1 Atspo11-1  double mutant indicated that chromosome fragmentation in  Atmnd1  was suppressed by loss of  Atspo11-1 . Fluorescence in situ hybridization (FISH) analysis showed that homologous pairing failed to occur and homologues remained apart throughout meiosis. At MND1  showed strong expression in meiocytes as revealed by RNA in situs.    Conclusion  We conclude that At MND1  is required for homologous pairing and is likely to play a role in the repair of DNA double strand breaks during meiosis in  Arabidopsis , thus showing conservation of function with that of  MND1  during meiosis in yeast.

AtMND1 is required for homologous pairing during meiosis in Arabidopsis