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Background  Autonomously Replicating Sequences ( ARS ) in  S. cerevisiae  serve as origins of DNA replication or as components of  cis -acting silencers, which impose positional repression at the mating type loci and at the telomeres. Both types of  ARS  can act as replicators or silencers, however it is not clear how these quite diverse functions are executed. It is believed that all  ARS  contain a core module of an essential  ARS  Consensus Sequence ( ACS ) and a non-essential  B1  element.    Results  We have tested how the  B1  elements contribute to the silencer and replicator function of  ARS . We report that the  ACS-B1  orientation of  ARS  has a profound effect on the levels of gene silencing at telomeres. We also report that the destruction of the canonical  B1  elements in two silencer  ARS  ( ARS317  and  ARS319 ) has no effect on their silencer and replicator activity.    Conclusions  The observed orientation effects on gene silencing suggest that  ARSs  can act as both proto-silencers and as insulator elements. In addition, the lack of  B1  suggests that the  ACS-B1  module could be different in silencer and replicator  ARS .

Directional telomeric silencing and lack of canonical B1 elements in two silencer Autonomously Replicating Sequences in S. cerevisiae