English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Bacteriocins are antimicrobial proteins and peptides ribosomally synthesized by some bacteria which can effect both intraspecies and interspecies killing.    Results   Moraxella catarrhalis  strain E22 containing plasmid pLQ510 was shown to inhibit the growth of  M. catarrhalis  strain O35E. Two genes ( mcbA  and  mcbB ) in pLQ510 encoded proteins predicted to be involved in the secretion of a bacteriocin. Immediately downstream from these two genes, a very short ORF ( mcbC ) encoded a protein which had some homology to double-glycine bacteriocins produced by other bacteria. A second very short ORF ( mcbI ) immediately downstream from  mcbC  encoded a protein which had no significant similarity to other proteins in the databases. Cloning and expression of the  mcbI  gene in  M. catarrhalis  O35E indicated that this gene encoded the cognate immunity factor. Reverse transcriptase-PCR was used to show that the  mcbA ,  mcbB ,  mcbC , and  mcbI  ORFs were transcriptionally linked. This four-gene cluster was subsequently shown to be present in the chromosome of several  M. catarrhalis  strains including O12E. Inactivation of the  mcbA ,  mcbB , or  mcbC  ORFs in  M. catarrhalis  O12E eliminated the ability of this strain to inhibit the growth of  M. catarrhalis  O35E. In co-culture experiments involving a  M. catarrhalis  strain containing the  mcbABCI  locus and one which lacked this locus, the former strain became the predominant member of the culture after overnight growth in broth.    Conclusion  This is the first description of a bacteriocin and its cognate immunity factor produced by  M. catarrhalis . The killing activity of the McbC protein raises the possibility that it might serve to lyse other  M. catarrhalis  strains that lack the  mcbABCI  locus, thereby making their DNA available for lateral gene transfer.

Identification of a bacteriocin and its cognate immunity factor expressed by Moraxella catarrhalis