English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background   mce3  is one of the four virulence-related  mce  operons of  Mycobacterium tuberculosis . In a previous work we showed that the overexpression of Mce3R in  Mycobacterium smegmatis  and  M. tuberculosis  abolishes the expression of  lacZ  fused to the  mce3  promoter, indicating that Mce3R represses  mce3  transcription.    Results  We obtained a knockout mutant strain of  M. tuberculosis  H37Rv by inserting a hygromycin cassette into the  mce3R  gene. The mutation results in a significant increase in the expression of  mce3  genes either  in vitro  or in a murine cell macrophages line as it was determined using promoter- lacZ  fusions in  M. tuberculosis . The abundance of  mce1 ,  mce2  and  mce4  mRNAs was not affected by this mutation as it was demonstrated by quantitative RT-PCR. The  mce3R  promoter activity in the presence of Mce3R was significantly reduced compared with that in the absence of the regulator, during the  in vitro  culture of  M. tuberculosis .    Conclusion  Mce3R repress the transcription of  mce3  operon and self regulates its own expression but does not affect the transcription of  mce1 ,  mce2  and  mce4  operons of  M. tuberculosis .

Study of the role of Mce3R on the transcription of mce genes of Mycobacterium tuberculosis