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Background  The  Brucella  genome contains an insertion sequence (IS) element called IS 711  or IS 6501 , which is specific to the genus. The copy number of IS 711  varies in the genome of the different  Brucella  species, ranging from 7 in  B. abortus ,  B. melitensis  and  B. suis  to more than 30 in  B. ovis  and in  Brucella  strains isolated from marine mammals. At present, there is no experimental evidence of transposition of IS 711 , but the occurrence of this element with a high copy number in some species, and the isolation of  Brucella  strains with "ectopic" copies of IS 711  suggested that this IS could still transpose.    Results  In this study we obtained evidence of transposition of IS 711  from the  B. ovis  and  B. pinnipedialis  chromosomes by using the "transposon trap" plasmid pGBG1. This plasmid expresses resistance to tetracycline only if the repressor gene that it contains is inactivated. The strains  B. melitensis  16 M,  B. abortus  RB51,  B. ovis  BOC22 (field strain) and  B. pinnipedialis  B2/94, all containing the plasmid pGBG1, were grown in culture media with tetracycline until the appearance of tetracycline resistant mutants (Tc R ). Tc R  mutants due to IS 711  transposition were only detected in  B. ovis  and  B. pinnipedialis  strains.    Conclusion  Four different copies of IS 711  were found to transpose to the same target sequence in the plasmid pGBG1. This demonstrated that IS 711  are active  in vivo , specially in  Brucella  species with a high number of IS 711  copies as  B. ovis  and  B. pinnipedialis .

Demonstration of IS711 transposition in Brucella ovis and Brucella pinnipedialis