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Abstract Background <it>Shigella </it>is the etiological agent of shigellosis, a disease responsible for more than 500,000 deaths of children per year, in developing countries. These pathogens colonize the intestinal colon, invade, spreading to the other enterocytes. Breastfeeding plays a very important role in protecting infants from intestinal infections. Amongst milk compounds, glycosylated proteins prevent the adhesion of many enteropathogens <it>in vitro</it>. The aim of this work was to determine the effect of human milk proteins on the colonization potential of <it>Shigella dysenteriae, S. flexneri and S. sonnei</it>. To fulfill this purpose, pooled milk samples from five donors, were fractionated by gel filtration and affinity chromatography. Using tissue culture, the milk fractions obtained were tested in <it>Shigella </it>adhesion and invasion assays. Results Our revealed showed that both adhesion and invasion of <it>Shigella </it>species were inhibited by low concentration of secretory immunoglobulin A, lactoferrin and free secretory component. This work also showed that, these proteins bind to superficial and whole-cell <it>Shigella </it>proteins. Conclusions Our findings suggest that human milk may act inhibiting adhesion and, consequently, invasion of <it>Shigella</it>, thereafter preventing shigellosis in infants.</p

In vitro adhesion and invasion inhibition of Shigella dysenteriae, Shigella flexneri and Shigella sonnei clinical strains by human milk proteins.