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Abstract Background The gene encoding the inorganic pyrophosphatase (PPase) of the intracellular pathogen <it>Legionella pneumophila</it> is induced during intracellular infection, but is constitutively expressed in <it>Eschericia coli.</it> The causative agent of tuberculosis, <it>Mycobacterium tuberculosis,</it> contains a well conserved copy of PPase. We sought to determine if expression of the <it>M. tuberculosis</it> PPase is regulated by the intracellular environment. Results A strain of <it>Mycobacterium bovis</it> bacille Calmette-Guérin (BCG) was constructed in which the <it>Aequoria victoria</it> green fluorescent protein (GFP) is controlled by the promoter of the <it>M. tuberculosis ppa</it> gene. After prolonged exposure of the recombinant BCG strain within murine bone-marrow-derived macrophages, there was no observed increased activity of the <it>ppa</it> promoter. Furthermore, there was no change in promoter activity after exposure to various stress stimuli such as reduced pH, osmotic shock, nutrient limitation or oxidative stress. Conclusions These results suggest that macrophage induction of <it>ppa</it> is not a general phenomenon among intracellular pathogens.</p

Analysis of stress- and host cell-induced expression of the Mycobacterium tuberculosis inorganic pyrophosphatase.