English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background:  Duplicated genes are common in vertebrate genomes. Their persistence is assumed to be either a consequence of gain of novel function (neofunctionalisation) or partitioning of the function of the ancestral molecule (sub-functionalisation). Surprisingly few studies have evaluated the extent of such modifications despite the numerous duplicated receptor and ligand genes identified in vertebrate genomes to date. In order to study the importance of function in the maintenance of duplicated genes, sea bream ( Sparus auratus ) PAC 1  receptors, sequence homologues of the mammalian receptor specific for PACAP (Pituitary Adenylate Cyclase-Activating Polypeptide), were studied. These receptors belong to family 2 GPCRs and most of their members are duplicated in teleosts although the reason why both persist in the genome is unknown.    Results:  Duplicate sea bream PACAP receptor genes (sbPAC 1 A and sbPAC 1 B), members of family 2 GPCRs, were isolated and share 77% amino acid sequence identity. RT-PCR with specific primers for each gene revealed that they have a differential tissue distribution which overlaps with the distribution of the single mammalian receptor. Furthermore, in common with mammals, the teleost genes undergo alternative splicing and a PAC 1 Ahop1 isoform has been characterised. Duplicated orthologous receptors have also been identified in other teleost genomes and their distribution profile suggests that function may be species specific. Functional analysis of the paralogue sbPAC 1 s in Cos7 cells revealed that they are strongly stimulated in the presence of mammalian PACAP 27  and PACAP 38  and far less with VIP (Vasoactive Intestinal Peptide). The sbPAC 1  receptors are equally stimulated (LOG EC50  values for maximal cAMP production) in the presence of PACAP 27  (-8.74 ± 0.29 M and -9.15 ± 0.21 M, respectively for sbPAC 1 A and sbPAC 1 B, P > 0.05) and PACAP 38  (-8.54 ± 0.18 M and -8.92 ± 0.24 M, respectively for sbPAC 1 A and sbPAC 1 B, P > 0.05). Human VIP was found to stimulate sbPAC 1 A (-7.23 ± 0.20 M) more strongly than sbPAC 1 B (-6.57 ± 0.14 M, P < 0.05) and human secretin (SCT), which has not so far been identified in fish genomes, caused negligible stimulation of both receptors.    Conclusion:  The existence of functionally divergent duplicate sbPAC 1  receptors is in line with previously proposed theories about the origin and maintenance of duplicated genes. Sea bream PAC 1  duplicate receptors resemble the typical mammalian PAC 1 , and PACAP peptides were found to be more effective than VIP in stimulating cAMP production, although sbPAC 1 A was more responsive for VIP than sbPAC 1 B. These results together with the highly divergent pattern of tissue distribution suggest that a process involving neofunctionalisation occurred after receptor duplication within the fish lineage and probably accounts for their persistence in the genome. The characterisation of further duplicated receptors and their ligands should provide insights into the evolution and function of novel protein-protein interactions associated with the vertebrate radiation.

Persistence of duplicated PAC1 receptors in the teleost, Sparus auratus