English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The great variety in sequence, length, complexity, and abundance of satellite DNA has made it difficult to ascribe any function to this genome component. Recent studies have shown that satellite DNA can be transcribed and be involved in regulation of chromatin structure and gene expression. Some satellite DNAs, such as the  pDo500  sequence family in  Dolichopoda  cave crickets, have a catalytic hammerhead (HH) ribozyme structure and activity embedded within each repeat.    Results  We assessed the phylogenetic footprints of the HH ribozyme within the  pDo500  sequences from 38 different populations representing 12 species of  Dolichopoda . The HH region was significantly more conserved than the non-hammerhead (NHH) region of the  pDo500  repeat. In addition, stems were more conserved than loops. In stems, several compensatory mutations were detected that maintain base pairing. The core region of the HH ribozyme was affected by very few nucleotide substitutions and the cleavage position was altered only once among 198 sequences. RNA folding of the HH sequences revealed that a potentially active HH ribozyme can be found in most of the  Dolichopoda  populations and species.    Conclusions  The phylogenetic footprints suggest that the HH region of the  pDo500  sequence family is selected for function in  Dolichopoda  cave crickets. However, the functional role of HH ribozymes in eukaryotic organisms is unclear. The possible functions have been related to  trans  cleavage of an RNA target by a ribonucleoprotein and regulation of gene expression. Whether the HH ribozyme in  Dolichopoda  is involved in similar functions remains to be investigated. Future studies need to demonstrate how the observed nucleotide changes and evolutionary constraint have affected the catalytic efficiency of the hammerhead.

bmc evolutionary biology

Phylogenetic footprinting of non-coding RNA: hammerhead ribozyme sequences in a satellite DNA family of Dolichopodacave crickets (Orthoptera, Rhaphidophoridae)