English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  Insect cells can serve as host systems for the recombinant expression of eukaryotic proteins. Using this platform, the controlled expression of  15 N/ 13 C labelled proteins requires the analysis of incorporation paths and rates of isotope-labelled precursors present in the medium into amino acids. For this purpose,  Spodoptera frugiperda  cells were grown in a complex medium containing [U- 13 C 6 ]glucose. In a second experiment, cultures of  S. frugiperda  were grown in the presence of  15 N-phenylalanine.    Results  Quantitative NMR analysis showed incorporation of the proffered [U- 13 C 6 ]glucose into the ribose moiety of ribonucleosides (40 – 45%) and into the amino acids, alanine (41%), glutamic acid/glutamine (C-4 and C-5, 30%) and aspartate/asparagine (15%). Other amino acids and the purine ring of nucleosides were not formed from exogenous glucose in significant amounts (> 5%). Prior to the incorporation into protein the proffered  15 N-phenylalanine lost about 70% of its label by transamination and the labelled compound was not converted into tyrosine to a significant extent.    Conclusion  Growth of  S. frugiperda  cells in the presence of [U- 13 C 6 ]glucose is conducive to the fractional labelling of ribonucleosides, alanine, glutamic acid/glutamine and aspartic acid/asparagine. The isotopolog compositions of the ribonucleosides and of alanine indicate considerable recycling of carbohydrate intermediates in the reductive branch of the pentose phosphate pathway. The incorporation of  15 N-labelled amino acids may be hampered by loss of the  15 N-label by transamination.

Studies of the intermediary metabolism in cultured cells of the insect Spodoptera frugiperda using 13C- or 15N-labelled tracers