English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

We aimed to develop a model for studying membrane leakiness. A microdialysis technique was used to investigate rubidium-86 ( 86 Rb) uptake in suspended human erythrocytes in vitro, with the aim of later applying the technique to in vivo studies. Suspensions were prepared from washed erythrocytes and  86 Rb administered directly or via the microdialysis probe. The effects on  86 Rb uptake of varying the haematocrit were measured. Erythrocytes were also treated with the K+ ionophore valinomycin or the Na+/K+-ATPase inhibitor ouabain. The effects on  86 Rb uptake, microdialysate content of lactate and pyruvate, and erythrocyte content of 2,3-bisphosphoglycerate (2,3-BPG) were measured. Valinomycin dissipates the potassium gradient and activates Na + /K + -ATPase, demonstrated by decreased erythrocyte  86 Rb uptake with increasing concentrations of valinomycin. This increased ion pump activity enhanced glycolysis, which was demonstrated by accumulation of pyruvate and lactate due to enhanced consumption of 2,3-BPG. The microdialysis technique is appropriate for in vitro studies of ion fluxes across cellular membranes.

Rubidium-86 Uptake and Energy Metabolism in Suspended Human Erythrocytes Monitored by Microdialysis