English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Objectives   Clinical disorders occurring in HIV-infected patients on antiretroviral therapy (ART) have been linked to mitochondrial dysfunction, for example, lactic acidosis and lipodystrophy. Mitochondrial membrane potential (ΔΨ m ) is the most direct measure of the state of energization of the mitochondria. We analysed ΔΨ m  of peripheral blood mononuclear cells (PBMCs) in HIV-negative, healthy subjects ( n=8), HIV-infected, treatment-naive patients ( n=30), and HIV-infected patients on ART ( n=58). The influence of ART was analysed in six patients who started their first regimen.Methods   The ΔΨ m  of PBMC was measured by flow cytometry using the dye JC-1.Results   The ΔΨ m  was significantly lower in HIV-infected patients than in HIV-negative controls. This difference was detected in both treated ( P=0.0001) and untreated patients ( P=0.001). The ΔΨ m  of PBMCs was highly correlated with CD4 +  T-cell count in therapy-naive patients ( P=0.002, r=0.546) and in treated patients ( P=0.028, r=0.288). The ΔΨ m  increased significantly in therapy-naive patients after starting ART ( P=0.001). Patients with lipoatrophy had significantly lower ΔΨ m  than patients without lipodystrophy or with lipohypertrophy ( P=0.023).Conclusions   In HIV-infected persons ΔΨ m  is significantly reduced. Patients with lipoatrophy have significantly reduced ΔΨ m . This is the first study showing that the ΔΨ m  of PBMCs is highly correlated with CD4 +  T-cell count in HIV infection.

The Influence of HIV Infection and Antiretroviral Therapy on the Mitochondrial Membrane Potential of Peripheral Mononuclear Cells