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The correlation between the antimicrobic standard disk agar diffusion procedure and a veterinary antimicrobic breakpoint minimal inhibitory concentration system was evaluated. Bacterial isolates representing 5 different genera were tested against 15 antimicrobics. There were 3,795 tests performed; 77.3% of the test results were in agreement, 22.6% were in disagreement. Thirty-one percent of the conflicting results were due to the organism being intermediate on the standard disk agar diffusion procedure. Results suggest that the breakpoint system needs more than 1 antimicrobic dilution per antimicrobic, or a change in some of the single dilutions in the breakpoint system would eliminate some of these discrepancies. Another possibility would be to utilize more than 1 panel design, i.e., 1 for gram-positive organisms, 1 for gram-negative organisms, and 1 for fastidious organisms.

journal of veterinary diagnostic investigation

Comparison of a Veterinary Breakpoint Minimal Inhibitory Concentration System and a Standardized Disk Agar Diffusion Procedure for Antimicrobic Susceptibility Testing