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In this experiment, CO was used as a gas mixture in a reversible relationship with O 2 . CO was added in a gas form mixed with O 2 . An isolated donor rat heart was obtained, exposed to a gas mixture such as PO 2  = 1,800 hPa and PCO = 200 hPa, and PO 2  = 1,000 hPa and PCO = 1,000 hPa in a 2 ATA high-pressure chamber and preserved in a refrigerator at 4°C. This report demonstrates that significant reproducibility has been verified. The heart was removed from the refrigerator 24 h later and heterotopic heart transplantation was performed in the right neck of a recipient rat and the pulsating of the transplanted heart was detected by an electrocardiogram.

Study on the Preservation with CO (PCO = 200–2,000 hPa), Resuscitation, and Heterotopic Transplantation of an Isolated Rat Heart