Open AccessSerum microRNA-92 expression in patients with ovarian epithelial carcinoma
Author(s) -
Fengjun Guo,
Jingyan Tian,
Lin Yang,
Yishuai Jin,
Ling Wang,
Manhua Cui
Publication year - 2013
Publication title -
journal of international medical research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.421
H-Index - 57
eISSN - 1473-2300
pISSN - 0300-0605
DOI - 10.1177/0300060513487652
Subject(s) - medicine , microrna , biomarker , clinical significance , ovarian cancer , oncology , ovarian carcinoma , epithelial ovarian cancer , stage (stratigraphy) , carcinoma , lymph node , real time polymerase chain reaction , reverse transcription polymerase chain reaction , cancer , pathology , messenger rna , gene , biology , paleontology , biochemistry
Objectives Studies have reported the significance of circulating microRNAs (miRNAs) as biochemical markers of cancer. Few reports demonstrate the significance of miRNAs in the serum of patients with ovarian epithelial carcinoma (EOC). The present study prospectively investigated the status of miRNA-92 (miR-92) in the serum of EOC patients and healthy controls, and examined the potential usefulness of this molecule as a biomarker for EOC diagnosis.Methods Levels of miR-92 in serum samples from EOC patients and controls were measured by quantitative real time reverse transcription–polymerase chain reaction (RT–PCR). Associations between serum miR-92 concentrations and clinical parameters were assessed by Fisher’s exact probability test.Results The serum concentration of miR-92 in EOC patients ( n = 50) was significantly higher than in controls ( n = 50). There were significant correlations between miR-92 expression and regional lymph node involvement and clinical stage of the tumour. There was no significant association between expression of miR-92 and age.Conclusion The detection of miR-92 levels in the serum might serve as a new tumour biomarker in the diagnosis and assessment of prognosis of EOC.