Open AccessbFGF Redox Signaling in Hypopharyngeal Cancer
Author(s) -
Fennessy Brendan G.,
Woolley John F.,
Cotter Thomas G.
Publication year - 2012
Publication title -
otolaryngology–head and neck surgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.232
H-Index - 121
eISSN - 1097-6817
pISSN - 0194-5998
DOI - 10.1177/0194599812451426a70
Subject(s) - basic fibroblast growth factor , fibroblast growth factor receptor , cancer research , fibroblast growth factor , apoptosis , signal transduction , cell growth , microbiology and biotechnology , reactive oxygen species , proliferating cell nuclear antigen , cancer cell , biology , chemistry , receptor , growth factor , cancer , medicine , biochemistry
Objective Examine the role of basic fibroblast growth factor (bFGF) in the generation of reactive oxygen species (ROS) in FaDu pharyngeal carcinoma cell lines. Evaluate the consequences of disrupting bFGF mediated signaling in tumor cells. Method Basic science study of hypopharyngeal carcinoma from 2010 to 2012. The study subjects were FaDu cell lines. Setting: In vitro. Interventions: Disrupting bFGF signaling. Outcomes Measured: ROS generation, apoptosis. Results bFGF and fibroblast growth factor receptor are co‐expressed in FaDu squamous cell carcinoma cells. ROS generation in FaDu cells takes place in response to bFGF stimulation, and is inhibited with a FGF receptor inhibitor, in a NADPH‐oxidase dependent and cyclooxygenase‐independent manner. Disrupting bFGF‐FGFR signaling results in an early strong apoptotic stimulus involving caspase‐3, ‐9, and poly ADP ribose polymerase. Conclusion bFGF‐mediated signaling in hypopharyngeal cancer has an important role in cell survival and proliferation.