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We developed a binary gradient high-performance liquid chromatography (HPLC) method for measuring HbA 2  in whole blood samples using a Pharmacia Mono Q column (1 mL) and measurement at 415 nm. The assay requires a simple lysis and centrifugation step before injection onto the column. We found good agreement of results between the HPLC method and the Helena column chromatography method. The within batch precision was 2·6% and between batch precision was 4·6%. We found that using 30 mM Tris buffers (pH 7·8) with a sodium chloride gradient resulted in short analysis times and good chromatographic separation of HbA 2 , HbS and HbA. We conclude that this is a robust assay for the diagnosis of β-thalassaemia.

A Rapid Anion Exchange High-Performance Liquid Chromatography Method for the Measurement of HbA2 in Whole Blood

A Rapid Anion Exchange High-Performance Liquid Chromatography Method for the Measurement of HbA₂ in Whole Blood