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Summary A method of acetate determination by gas phase chromatography using a porous polymer stationary phase is reported that is suitable for use with aqueous and plasma samples. It is linear up to 100 mmol/l and has a coefficient of variation of less than 4 % for acetate values of greater than 1 mmol/l. The recovery of acetate from plasma is 92 % and sample retention time is 3 minutes. The reference range of plasma acetate in a group of 40 apparently healthy subjects was from less than 0·1 to 0·35 mmol/l. The frequently encountered problem of adsorption and ghosting of volatile fatty acids is overcome without the addition of formic acid vapour to the carrier gas.

Measurement of Acetate in Aqueous Solutions and Plasma by Gas Phase Chromatography using a Porous Polymer Stationary Phase