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Micronuclei are structures composed by chromatin material contained in the nuclear envelope and smaller than the main nucleus. Micronucleus test has been widely used in the genotoxic potential evaluation of different compounds. Although a few report concerning on the mechanism of micronucleus genesis, its DNA sequences content and the cell cycle phase when they arise. The aim of this work was to analyze the kinetic of micronuclei in A549 cells from human lung carcinoma submitted to cell cycle synchronization. After double blocking by fetal bovine serum deprivation and vincristine treatment, micronucleus formation was monitored with a S-phase marker. The results have showed that both in the mitosis and in the interphasic phase, micronuclei may arise and they were able to replicate its DNA. This process seemed to be independent of main nucleus. Cellular ability to escape from double blocking suggests that mitotic spindle checkpoint in A549 is not functional. Moreover, EGFR sequences were detected into the micronucleus. It is important to elucidate the micronucleus meaning to describe precisely its association with elimination of oncogene or other amplified sequences from the tumor cells.

Micronúcleos em células tumorais: biologia e implicações para a tumorigênese