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Although deproteinization using dialysis should not, in principle, suffer from these disadvantages, the resulting dialysate would contain sub-analysable concentrations of amino-acids. However, the high sensitivity ofpre-column o-phthalaldehyde/2-mercaptoethanol (OPA/MCE) derivatization and reversed phase high pressure liquid chromatography (HPLC) methods [2-4] permitted the examination of the potential of this deproteinization technique.

journal of automatic chemistry

Automated preparation of biological samples prior to high pressure liquid chromatography: Part I- The use of dialysis for deproteinizing serum for amino-acid analysis