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Plasma membrane Na + /Ca 2+  exchanger-1 (NCX1) helps regulate the cytosolic Ca 2+  concentration ([Ca 2+ ] CYT ) in arterial myocytes. NCX1 mediates both Ca 2+  entry and exit and tends to promote net Ca 2+  entry in partially constricted arteries. Mean blood pressure (telemetry) is elevated by ≈10 mmHg in transgenic (TG) mice that overexpress NCX1 specifically in smooth muscle. We tested the hypothesis that NCX1 overexpression mediates Ca 2+  gain and elevated [Ca 2+ ] CYT  in exposed femoral arteries that also express the Ca 2+  biosensor exogenous myosin light chain kinase. [Ca 2+ ] CYT  and the NCX1-dependent (SEA0400-sensitive) component, ≈15% of total basal constriction in controls, were increased in TG arteries, but constrictions to phenylephrine and ANG II were comparable in TG and control arteries. Normalized phenylephrine dose-response curves and constriction to 30 and 300 ng/kg iv ANG II were virtually identical in control and TG arteries. ANG II-evoked constrictions, superimposed on elevated basal tone, accounted for the larger blood pressure responses to ANG II in TG arteries. TG and control mouse arteries fit the same pCa-constriction relationship over a wide range of pCa (≈125–500 nM). Vasodilation to acetylcholine, normalized to passive diameter, was also comparable in TG and control arteries, implying normal endothelial function. TG artery Na +  nitroprusside (nitric oxide donor)-induced dilations were, however, shifted to lower Na +  nitroprusside concentrations, indicating that TG myocyte vasodilator mechanisms were augmented. Maximum arterial dilation was comparable in TG and control mice, although passive diameter was ≈6–7% smaller in TG mice. The changes in TG arteries were apparently largely functional rather than structural, despite the congenital hypertension.    NEW &amp; NOTEWORTHY Smooth muscle Na + /Ca 2+  exchanger-1 transgene overexpression (TG mice) increases femoral artery basal cytosolic Ca 2+  concentration ([Ca 2+ ] CYT ) and tone in vivo and raises blood pressure. Arterial constriction to phenylephrine and angiotensin II are normal but superimposed on the augmented basal [Ca 2+ ] CYT  and tone (constriction) in TG mouse arteries. Similar effects in resistance arteries would explain the elevated blood pressure. Acetylcholine-induced vasodilation is unimpaired, implying a normal endothelium, but TG arteries are hypersensitive to sodium nitroprusside.

Na+/Ca2+ exchanger overexpression in smooth muscle augments cytosolic Ca2+ in femoral arteries of living mice

Na⁺/Ca²⁺ exchanger overexpression in smooth muscle augments cytosolic Ca²⁺ in femoral arteries of living mice