Using IRAP markers for analysis of genetic variability in populations of resource and rare species of plants
Author(s) -
С. В. Боронникова,
Ruslan Kalendar
Publication year - 2010
Publication title -
russian journal of genetics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.208
H-Index - 24
eISSN - 1608-3369
pISSN - 1022-7954
DOI - 10.1134/s1022795410010060
Subject(s) - retrotransposon , biology , genetic diversity , genetics , evolutionary biology , long terminal repeat , genome , transposable element , gene , population , demography , sociology
Species-specific LTR retrotransposons were first cloned in five rare relic species of drug plants located in the Perm’ region. Sequences of LTR retrotransposons were used for PCR analysis based on amplification of repeated sequences from LTR or other sites of retrotransposons (IRAP). Genetic diversity was studied in six populations of rare relic species of plants Adonis vernalis L. by means of the IRAP method; 125 polymorphic IRAP-markers were analyzed. Parameters for DNA polymorphism and genetic diversity of A. vernalis populations were determined.
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