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ABSTRACT  Vsr217 is a small RNA from  Vibrio tasmaniensis  LGP32, a pathogen associated with mortality events affecting juvenile oysters. The  vsr217  gene is located within the 5′ untranslated region (UTR) of  malK , encoding the ATPase component of the maltose importer, and is conserved within the genus  Vibrio . In the presence of maltose,  vsr217  is regulated by MalT, the positive regulator of the maltose regulon.  vsr217  is required in  cis  for the full expression of  malK . In addition, Vsr217 acts in  trans  to downregulate the expression of  fbp  encoding fructose-1,6-bisphosphatase, an enzyme involved in gluconeogenesis. Thus, in the presence of maltose, the induction of Vsr217 is expected to promote glycolysis by negatively regulating the expression of a key enzyme of gluconeogenesis.   IMPORTANCE  Juvenile pacific oysters have been subject in recent years to summer mortality episodes with deep economic consequences. The pathogen  Vibrio tasmaniensis  has been associated with such mortality events. For bacterial pathogens, survival within the host requires profound metabolic adaptations according to available resources. All kinds of regulatory elements, including noncoding RNAs, orchestrate this response. Oysters are rich in glycogen, a precursor of maltose, and we previously reported that  V. tasmaniensis  maltose-regulated genes are strongly induced during oyster infection. Here, we report the dual mechanism by which a small regulatory RNA, generated from the 5′ untranslated region of a gene belonging to the maltose regulon, acts both in  cis  and  trans . In  cis , it stimulates growth on maltose, and in  trans , it downregulates the expression of a gene associated with gluconeogenesis, thus coordinating maltose utilization with central carbon metabolism.

A Small Regulatory RNA Generated from the malK 5′ Untranslated Region Targets Gluconeogenesis in Vibrio Species