English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

One striking feature of viruses with RNA genomes is the modification of the host membrane structure during early infection. This process requires both virus- and host-encoded proteins; however, the host factors involved and their role in this process remain largely unknown. On infection withTobacco mosaic virus (TMV), a positive-strand RNA virus, the filamentous and tubular endoplasmic reticulum (ER) converts to aggregations at the early stage and returns to filamentous at the late infectious stage, termed the ER transition. Also, membrane- or vesicle-packaged viral replication complexes (VRCs) are induced early during infection. We used microarray assays to screen theArabidopsis thaliana gene(s) responding to infection with TMV in the initial infection stage and identified anArabidopsis gene,PAP85 (annotated as a vicilin-like seed storage protein), with upregulated expression during 0.5 to 6 h of TMV infection. TMV accumulation was reduced inpap85 -RNA interference (RNAi)Arabidopsis and restored to wild-type levels when PAP85 was overexpressed inpap85 -RNAiArabidopsis . We did not observe the ER transition in TMV-infectedPAP85 -knockdownArabidopsis protoplasts. In addition, TMV accumulation was reduced inPAP85 -knockdown protoplasts. VRC accumulation was reduced, but not significantly (P = 0.06), inPAP85 -knockdown protoplasts. Coexpression of PAP85 and the TMV main replicase (P126), but not their expression alone inArabidopsis protoplasts, could induce ER aggregations.

A Vicilin-Like Seed Storage Protein, PAP85, Is Involved in Tobacco Mosaic Virus Replication