Open AccessUse of Specific rRNA Oligonucleotide Probes for Microscopic Detection of Mycobacterium tuberculosis in Culture and Tissue Specimens
Author(s) -
Allison L. St. Amand,
Daniel N. Frank,
Mary Ann De Groote,
Randall J. Basaraba,
Ian M. Orme,
Norman R. Pace
Publication year - 2005
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.43.10.5369-5371.2005
Subject(s) - mycobacterium tuberculosis , tuberculosis , oligonucleotide , mycobacterium , biology , ribosomal rna , oligomer restriction , microbiology and biotechnology , pathology , medicine , genetics , dna , gene
Mycobacterium tuberculosis infections are a major global health problem. Fast and accurate detection ofM. tuberculosis is clearly needed at both the clinical level and the research level. We report a rapid and reliable in situ hybridization technique using rRNA oligonucleotide probes for the identification ofM. tuberculosis in tissues and cultures.