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A nucleotide transversion from guanine to uracil in the 23S rRNA confers linezolid resistance. We describe a real-time PCR using two Taqman probes that detects a single mutated allele among the genomes of Enterococcus faecium and Enterococcus faecalis. Results were confirmed by a classical approach involving LabChip technology assayed with an Agilent Bioanalyzer 2100.

journal of clinical microbiology

Molecular Detection of Linezolid Resistance in <i>Enterococcus faecium</i> and <i>Enterococcus faecalis</i> by Use of 5′ Nuclease Real-Time PCR Compared to a Modified Classical Approach

Molecular Detection of Linezolid Resistance in Enterococcus faecium and Enterococcus faecalis by Use of 5′ Nuclease Real-Time PCR Compared to a Modified Classical Approach