Open AccessSelective Amplification of tyv (rfbE), prt (rfbS), viaB , and fliC Genes by Multiplex PCR for Identification of Salmonella enterica Serovars Typhi and Paratyphi A
Author(s) -
Kenji Hirose,
Kiyohiko Itoh,
Hiroshi Nakajima,
Takayuki Kurazono,
Masanori Yamaguchi,
Kazuo Moriya,
Takayuki Ezaki,
Yoshiaki Kawamura,
Kazumichi Tamura,
Haruo Watanabe
Publication year - 2002
Publication title -
journal of clinical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.349
H-Index - 255
eISSN - 1070-633X
pISSN - 0095-1137
DOI - 10.1128/jcm.40.02.633-636.2002
Subject(s) - serotype , multiplex polymerase chain reaction , salmonella typhi , biology , salmonella enterica , microbiology and biotechnology , salmonella , polymerase chain reaction , virology , multiplex , gene , bacteria , genetics , escherichia coli
The PCR primers for O, H, and Vi antigen genes,tyv (rfbE),prt (rfbS ),fliC-d, fliC-a , andviaB , were designed and used for the rapid identification ofSalmonella enterica serovars Typhi and Paratyphi A with multiplex PCR. The results showed that all the clinical isolates examined ofSalmonella serovars Typhi and Paratyphi A were accurately identified by this assay.