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The Oxoid combination disk method for detecting extended-spectrum beta-lactamases (ESBLs) depends on comparing the inhibition zones of cefpodoxime (10-microg) and cefpodoxime-plus-clavulanate (10- plus 1-microg) disks. The presence of clavulanate enlarged the zones for all of 180 ESBL-producing klebsiellae by &gt;/=5 mm, whereas zones for cefpodoxime-susceptible isolates and cefpodoxime-resistant isolates with AmpC and K1 beta-lactamases were enlarged by &lt;/=1 mm. Good discrimination was achieved with either the NCCLS or the British disk method.

Detection of Extended-Spectrum β-Lactamases in Klebsiellae with the Oxoid Combination Disk Method