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16S rRNA methyltransferases confer resistance to most aminoglycosides, but discriminating their activity from that of aminoglycoside-modifying enzymes (AMEs) is challenging using phenotypic methods. We demonstrate that arbekacin, an aminoglycoside refractory to most AMEs, can rapidly detect 16S methyltransferase activity inEnterobacteriaceae with high specificity using the standard disk susceptibility test.

Detecting 16S rRNA Methyltransferases in Enterobacteriaceae by Use of Arbekacin