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Suh, Byungse (University of Kansas, Lawrence),and J. M. Akagi . Pyruvate-carbon dioxide exchange reaction ofDesulfovibrio desulfuricans . J. Bacteriol.91: 2281–2285. 1966.—The pyruvate-CO2 exchange reaction, catalyzed byDesulfovibrio desulfuricans , required the presence of phosphate and coenzyme A. However, the requirement for phosphate disappeared when the concentration of coenzyme A was increased to a level of 3.8 × 10−3 m . Passing crude extracts through a diethylaminoethyl-cellulose column and an Amberlite CG-50 ion-exchange column, to remove ferredoxin and cytochromec 3 , resulted in a marked decrease in exchange activity; full activity was restored by the addition of ferredoxin or cytochromec 3 . Fe++ or Co++ stimulated the exchange of CO2 into pyruvate.

Pyruvate-Carbon Dioxide Exchange Reaction of Desulfovibrio desulfuricans