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Narkates, Annie J. (Southern Research Institute, Birmingham, Ala.),and Robert F. Pittillo . Influence of inoculum media on sensitivity ofEscherichia coli to azaserine. J. Bacteriol.90: 710–714. 1965.—Escherichia coli grown in a simple synthetic medium is more than 100-fold more sensitive to inhibition by azaserine thanE. coli grown in complex, undefined organic medium. The work reported here offers two explanations for this observed effect: (i) concentration within the cell from the complex medium of a specific azaserine-reversing metabolite (nicotinic acid), and (ii) stimulation by exogenous metabolite(s) of intracellular synthesis of azaserine-reversing agents.

Influence of Inoculum Media on Sensitivity of Escherichia coli to Azaserine