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phate were incubated at 37 C. At time intervals, samples of the incubation mixture were acidified, centrifuged, neutralized to pH 7.0 with dilute NaOH, and assayed for glucose-6-phosphate and fructose-6-phosphate (Racker and Schroeder, Arch. Biochem. Biophys. 74:326, 1958), inorganic phosphate (Fiske and SubbaRow, J. Biol. Chem. 66:375, 1925), and fructose 1,6 diphosphate (Racker, J. Biol. Chem. 167:843, 1947). The results are shown in Table 2, and indicate that for each mole of fructose-1 ,6-diphosphate which disappeared, 1 mole of inorganic phosphate and 1 mole of fructose-6-phosphate were formed. Various sugar phosphates were tested as substrates for the purified phosphatase. Glucose1-phosphate, glucose-6-phosphate, fructose-6TABLE 2. Stoichiometry of phosphatase reaction

SAFRANINE O: RELIABLE SELECTIVE DYE FOR CHARACTERIZATION OF BRUCELLA SUIS