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Shank , J. L. (Swift &amp; Co., Chicago, Ill.). Applications of the plastic film technique in the isolation and study of anaerobic bacteria. J. Bacteriol.86: 95–100. 1963.—The use of plastic films as oxygen barriers on the surface of agar pour plates, in conjunction with thioglycolate and other selective and differential agents, allows the primary isolation and enumeration of clostridia and other anaerobes. Quantitative studies reveal little if any inhibition of the test organisms under these conditions, and toxin production, where it occurs, is shown to be virtually unimpaired.

APPLICATIONS OF THE PLASTIC FILM TECHNIQUE IN THE ISOLATION AND STUDY OF ANAEROBIC BACTERIA