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In the course of attempts to effect intergeneric transduction by means of coliphage P1 (Bertani, J. Bacteriol. 62:293, 1951; Lennox, Virology 1:190, 1955), we observed that this phage can attack and kill many strains of Pseudomonas aeruginosa and Serratia marcescens. Bacterial killing is not accompanied by phage multiplication. Lysates of phage Plkc or its mutant Plkc vir, grown on either Shigella dysenteriae Sh or Escherichia coli K-12 derivatives, were tested by spotting loopfuls of serial dilutions on plates of complete or minimal medium agar seeded with a soft-agar layer containing enough test bacteria to give a continuous layer of growth. The plates were incubated at various temperatures from 20 to 42 C. Inhibition reactions, in the form of clear or partially clear areas, were obtained with 22 out of 47 strains of P. aeruginosa tested. Similar but less reproducible results were obtained by spot tests on plates inoculated by spreading bacteria on the agar surface. P. aeruginosa strain 1 [try] (Holloway), which

ABORTIVE INFECTION OF PSEUDOMONAS AERUGINOSA AND SERRATIA MARCESCENS WITH COLIPHAGE P1