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The techniques of ultrathin sectioning and electron microscopy have been applied to cultures or suspensions of mycobacteria (Brieger and Glauert, 1956; Toda et al., 1957; Shinohara et at., 1957; Shinohara et al., 1958). The provocative nature of the resultant observations was sufficient in itself to suggest the desirability of studying these organisms as they occur in tissues. This project seemed all the more worthwhile when it was realized that, although a wide variety of microorganisms have been examined using these techniques, only the work of Goodman and Moore (1956), Goodman et al. (1956), Meyer and Frank (1957), and Brieger and Glauert (1956) has considered, via this high resolution approach, the relationships between the host cell and the microorganism, and the fine structure of the microorganism under this more pertinent condition of growth in an infected cell.

AN ELECTRON MICROSCOPE STUDY OF THE DISPOSITION AND FINE STRUCTURE OF MYCOBACTERIUM LEPRAEMURIUM IN MOUSE SPLEEN