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Isolation of Vibrio fetus is desirable in making a positive diagnosis of vibriosis; however this is often made difficult by (1) the presence of other microorganisms, especially when liquid media are used, and (2) the failure of solid media to support growth of some strains on primary culture under the generally accepted method of incubation in jars in which 5 to 10 per cent of the air is replaced with carbon dioxide. Recent reports of Bryner and Frank (1955a) and Reich et al. (1956) show that growth of V. fetus of bovine origin is increased in an atmosphere in which the oxygen content is reduced below that of air. The relation of different concentrations of carbon dioxide and oxygen in the gaseous environment to growth remains to be determined. For this reason the effect of atmospheres containing various levels of carbon dioxide, hydrogen, nitrogen and oxygen on growth of V. fetus on blood agar was observed. The results are reported here.

EFFECT OF GASEOUS ENVIRONMENT ON GROWTH AND CATALASE CONTENT OF VIBRIO FETUS CULTURES OF BOVINE ORIGIN