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The numbers of fungi in the soil are usually determined by the plate method used for the determination of the number of bacteria. In view of the fact that the dilution used for the determination of bacteria is necessarily high, due to the large numbers of bacteria in the soil, the fungi are so diluted that very few appear on the plate: of a dozen plates prepared from the same soil, using the same dilution, three or four may be free from fungi entirely, three or four may have only one or two fungus colonies, while three or four may have several colonies, particularly in the case of humus-rich and acid soils. It has been pointed out by the author (1922 a) that the probable error involved in the determination of the numbers of fungi by this method is so great, as to make the results absolutely worthless. To reduce the variability of the numbers of fungi on the plate and thus obtain a low probable error, low dilutions have to be used, so as to have 30 to 100 fungus colonies developing on the plate; this would necessitate a dilution of only 500 to 2000 for an ordinary fertile soil. But, if the comon plate used for the determination of bacterial numbers is employed, so many bacteria will develop on the plate, as to prevent the development of most of the fungi. To obviate this difficulty, use was made of the fact that fungi can grow readily at a much higher acidity than the bacteria and actinomycetes. The author and others have long made use of the fact, that, when a culture of a fungus is wanted free from bacteria raisin

A METHOD FOR COUNTING THE NUMBER OF FUNGI IN THE SOIL