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Cultures. The species employed in this study were: Proteus vulgaris, strain no. 9920, obtained from the American Type Culture Collection; Proteus vulgaris, strain no. 193, from the laboratory stock collection of the Department of Bacteriology, University of Connecticut; and Proteus mirabilis, strain nos. 32,500 and 53,402, from the Storrs Agricultural Experiment Station. All cultures were examined for purity and were found to conform biochemically to descriptions in Bergey's Manual (Breed, et al., 1948), except that strain no. 193 failed to produce gas from carbohydrates. Irradiation equipment. Irradiation was performed by means of a Westinghouse sterilamp (WL-872-20) from which 95 per cent of ultraviolet radiation was in the region of 2537 A. The lamp was operated 15 minutes before exposing cells at a distance of 30 cm; the potential was maintained at 110 volts. By means of a Westinghouse sterilamp meter (SM-600), the intensity of radiant power at 2537 A was found to average 22 microwatts per cm2 at a distance of 1 meter. Considering the sterilamp tube as a finite line source, and assuming the applicability of the inverse square law, dosage at this distance was approximately 240 microwatt-minutes per cm2. Irradiation procedure. The irradiations were conducted at room temperature. Nutrient agar plates (1.8 per cent agar) were seeded with 0.1 ml of an 8 to 12 hour nutrient broth culture of each strain tested. The inoculum was spread evenly, leaving an unseeded margin one cm from the side of the dish. Duplicate, uncovered plates of each strain were exposed to radiation for periods of 8, 16, and 32 minutes. Separate pairs of plates were used for each of these exposure periods. After incubation of these plates at 37 C for 24 to 36 hours, an isolated colony was picked from the center of each plate to nutrient broth. The broth cultures were incubated 8 to 12 hours, seeded to agar plates, and irradiated again as before. The "substrains" which survived exposure for 8 minutes were subjected to repeated 8-minute exposures; those which survived 16-minute exposure, to repeated

EFFECT OF ULTRAVIOLET RADIATION ON PRODUCTION OF UREASE BY PROTEUS VULGARIS AND PROTEUS MIRABILIS