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The need for a chemically defined medium in which strains of Streptococcus lactis and Streptococcus cremoris would grow was felt in this laboratory while work was being done with bacteriophages active against strains of the lactic group of streptococci. Storrs and Anderson (1949) have reported that the medium of Niven (1944), modified by the substitution of lactose for glucose and the addition of phosphate for buffering, was inadequate for normal acid production by S. lactis. Supplementation with "yeastrel," with fish-meal digest, or with certain incompletely defined fractions of these products stimulated acid production. The present study describes the results obtained when various supplements were added to the medium of Niven (1944), which was found inadequate for the growth of 9 of 31 strains of S. lactis and all 22 strains of S. cremoris studied.

ACETATE AND OLEATE REQUIREMENTS OF THE LACTIC GROUP OF STREPTOCOCCI