Open AccessSecond-Site Suppressor Mutations of Inactivating Substitutions at Gly247 of the Tetracycline Efflux Protein, Tet(B)
Author(s) -
Cynthia A. Saraceni-Richards,
Stuart B. Levy
Publication year - 2000
Publication title -
journal of bacteriology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.652
H-Index - 246
eISSN - 1067-8832
pISSN - 0021-9193
DOI - 10.1128/jb.182.22.6514-6516.2000
Subject(s) - tetracycline , biology , efflux , suppressor , mutation , transmembrane protein , genetics , cytoplasm , microbiology and biotechnology , transmembrane domain , gene , antibiotics , receptor
An Asp or Asn substitution for Gly247 in transmembrane helix 8 (TM-8) of Tet(B), the tetracycline efflux protein, eliminated tetracycline resistance. Second site suppressor mutations which partially restored resistance were located in TM-5, -8, -10, or -11 or in cytoplasmic loop 8-9 or loop 10-11. These results indicate physical proximity or functional relationships between TM-8 and these other regions of Tet(B).