English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The ordered assembly of theCaulobacter crescentus flagellum is accomplished in part through the organization of the flagellar structural genes in a regulatory hierarachy of four classes. Class II genes are the earliest to be expressed and are activated at a specific time in the cell cycle by the CtrA response regulator. In order to identify gene products required for early events in flagellar assembly, we used the known phenotypes of class II mutants to identify new class II flagellar genes. In this report we describe the isolation and characterization of a flagellar gene,fliX . AfliX null mutant is nonmotile, lacks a flagellum, and exhibits a marked cell division defect. Epistasis experiments placedfliX within class II of the flagellar regulatory hierarchy, suggesting that FliX functions at an early stage in flagellar assembly. ThefliX gene encodes a 15-kDa protein with a putative N-terminal signal sequence. Expression offliX is under cell cycle control, with transcription beginning relatively early in the cell cycle and peaking inCaulobacter predivisional cells. Full expression offliX was found to be dependent onctrA , and DNase I footprinting analysis demonstrated a direct interaction between CtrA and thefliX promoter. ThefliX gene is located upstream and is divergently transcribed from the class III flagellar geneflgI , which encodes the basal body P-ring monomer. Analysis of thefliX-flgI intergenic region revealed an arrangement ofcis -acting elements similar to that of another set ofCaulobacter class II and class III flagellar genes,fliL-flgF , that is also divergently transcribed. In parallel with the FliL protein, FliX copurifies with the membrane fraction, and although its expression is cell cycle controlled, the protein is present throughout the cell cycle.

A Membrane-Associated Protein, FliX, Is Required for an Early Step in Caulobacter Flagellar Assembly