Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine | Zendy

S L Otten | Zendy; Mark A. Gallo | Zendy; Krishnamurthy Madduri | Zendy; X Liu | Zendy; C. Richard Hutchinson | Zendy

Open Access

Cloning and characterization of the Streptomyces peucetius dnmZUV genes encoding three enzymes required for biosynthesis of the daunorubicin precursor thymidine diphospho-L-daunosamine

Author(s) -

S L Otten,

Mark A. Gallo,

Krishnamurthy Madduri,

X Liu,

C. Richard Hutchinson

Publication year - 1997

Publication title -

journal of bacteriology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.652

H-Index - 246

eISSN - 1067-8832

pISSN - 0021-9193

DOI - 10.1128/jb.179.13.4446-4450.1997

Subject(s) - biology , daunorubicin , gene , thymidine , biochemistry , dna , biosynthesis , cloning (programming) , microbiology and biotechnology , genetics , leukemia , computer science , programming language

Characterization of the dnmZ, dnmU, and dnmV genes from the daunorubicin-producer Streptomyces peucetius by DNA sequence analysis indicated that these genes encode a protein of unknown function plus a putative thymidine diphospho-4-keto-6-deoxyglucose-3(5)-epimerase and thymidine diphospho-4-ketodeoxyhexulose reductase, respectively. Inactivation of each of the three genes by gene disruption and replacement in the wild-type strain demonstrated that all of them are required for daunosamine biosynthesis.

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